Abstract: In the spectral analysis experiments of ore composition, the ore powders should be tightly filled into the test carbon rod. To enhance the filling effect of micro powders, the two-dimensional vibrating filler method added by auxiliary medium was introduced for filling and compacting. The mechanical contact model of micro-powders was established by discrete element method, and the particle model of micro-powders was constructed and simulation by EDEM software. By considering the main parameters of vibration packing (vibration frequency and vibration time), the two-dimensional vibrating filling process of the mixture ore powders in the particle sizes of 50~150 μm was simulated by using agate ball as the auxiliary medium in the cavity volume of 1 cm³, when the vibration frequency was 60~80 Hz and the vibration time was 10~15 s; the influencing factors of vibration frequency and vibration time on the filling rate and packing density of micro-powders were analyzed and discussed; finally, the simulation was verified by vibratory packing experiment in the self-made table. The results show that the filling material is compacted in two-dimensional vibrating filler method added by auxiliary medium, and the filling quality can meet the test requirements. The validity of simulation model is verified by experiment, providing a reference for other powder fillers.