Abstract: Ti-based amorphous alloys exhibit the unique disordered structure, combining with the high specific strength, excellent corrosion resistance, and distinctive physicochemical characteristics, which endow them with broad application prospects in aerospace, biomedical engineering, and precision manufacturing. However, the limited glass-forming ability and room-temperature brittleness remain the key bottlenecks restricting the engineering applications of Ti-based amorphous alloys. The structural characteristics and the thermodynamic and kinetic conditions for formation of Ti-based amorphous alloys were summarized in this paper, and the detailed introduction to the principles and applicable scenarios of various preparation methods was provided, including melt spinning, copper mold suction casting, mechanical alloying, and powder metallurgy. On this basis, the effects of structural regulation approaches, such as post-heating crystallization, electropulsing treatment, and deep cryogenic cycling treatment, on the microstructure and mechanical properties were systematically discussed, and the central roles of free volume and shear transformation zones in plastic deformation were elucidated. Furthermore, the corrosion behavior and corrosion resistance mechanism of Ti-based amorphous alloys in acidic and physiological environments were introduced. Finally, focusing on the cutting-edge field of irradiation effects, the critical conditions for irradiation-induced crystallization, the regulation mechanism of irradiation on mechanical properties, and the unique surface morphology evolution behavior were thoroughly analyzed.